青云英语翻译

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该apoflavodoxin - FMN络合物在其完全氧化状态的解离常数在一个SMF 25分光荧光计(控创),在25 ( 0.1℃的黑暗中荧光计测定。激发是在445纳米,发射被记录在525nm处。

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Apoflavodoxin-FMN 配合物在其完全氧化状态中的解离常数在被确定了 fluorometrically SMF 25 分光-fluorimeter (Kontron) 25 (0.1 业主立案法团在黑暗中。激励是在 445 nm 和排放录在 525 nm。使用 FMN 是 > 95%纯根据反相高效液相色谱法。在一个典型的实验,1 毫升的 0.2 µ M FMN 磷酸酯钾 50 毫米,在 ph 值 7.0,被滴定与整除的 5 300 µ M apoflavodoxin (根据突变体) 在相同的缓冲区数。绑定到辅助因子蛋白的强烈消宿食 FMN 荧光发射。每个蛋白加入后,系统允许达

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apoflavodoxin-FMN复合体的离解常数在他们充分地被氧化的状态的在一个SMF 25 Spectro-氟量计(Kontron)被确定了荧光在25 (0.1 oC在黑暗中。励磁在445毫微米,并且放射被记录了在525毫微米。半新的FMN是>95%纯净根据反向阶段高性能液体色谱。在一个典型的实验, 1 mL 0.2 µM FMN在50 mM钾磷酸盐,酸碱度7.0,滴定了与整除数5-300 µM apoflavodoxin (根据突变体)在同一缓冲。

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aThe dissociation constants of the apoflavodoxin-FMN complexes in their fully oxidized state were determined fluorometrically in an SMF 25 Spectro- fluorimeter (Kontron) at 25 ( 0.1 oC in darkness. Excitation was at 445 nm, and the emission was recorded at 525 nm. The FMN used was >95% pure according to reverse-phase HP 正在翻译,请等待... [translate]